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pet-29b(+) vector  (Millipore)


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    Structured Review

    Millipore pet-29b(+) vector
    Pet 29b(+) Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet-29b(+)+vector/pet28a/pmc11211065-240-10-12
    Average 90 stars, based on 1 article reviews
    pet-29b(+) vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: A Drosophila melanogaster model for TMEM43-related arrhythmogenic right ventricular cardiomyopathy type 5.
    Article Snippet: A 402 bp sequence, corresponding to the loop encoding region (aa 34-168), was amplified by PCR and cloned into the pET-29b vector (Novagen, Madison, WI) to generate a His-tag fusion construct.

    Article Title: The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit
    Article Snippet: The PCR products were digested with Nde I and Xho I and inserted into pET-29b vector (Novagen); Escherichia coli BL21(DE3) pLysS cells were transformed with this vector.

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: The tyrosinase-like gene cDNA (Tlg-cDNA) insert was excised from pZL1 (Gibco BRL) with Eco RI and Hind III and recloned into the pET-29b vector (Novagen).

    Article Title: Species Specificity of Vaccinia Virus Complement Control Protein for the Bovine Classical Pathway Is Governed Primarily by Direct Interaction of Its Acidic Residues with Factor I
    Article Snippet: All the mutants were subcloned into the pET-29b vector (Novagen) and transformed into Escherichia coli BL21 cells for expression.

    Article Title: N-Terminal Segment of Tv CyP2 Cyclophilin from Trichomonas vaginalis Is Involved in Self-Association, Membrane Interaction, and Subcellular Localization
    Article Snippet: Tv CyP2 and Tv CyP2-∆N were cloned into the pET-29b vector (Novagen, Darmstadt, Germany) by using NdeI and XhoI restriction sites and were expressed in E. coli strain BL21(DE3) with an additional His-tag at the C-terminus [ ].

    Article Title: Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization
    Article Snippet: Full-length cDNA for NLP, Nph, and TAP/p32 (LD16456-, SD23244-, and LD29590-expressed sequences tags, respectively) were amplified by PCR and cloned in-frame with C-terminal 6-His tag into pET-29b vector (Novagen).

    Article Title: Functional analysis of the GmESR1 gene associated with soybean regeneration
    Article Snippet: The PCR products were digested with Bam HI and Hin dIII and were inserted into the pET-29b vector (EMD Millipore, USA).

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis.
    Article Snippet: The gene encoding Myb135–141 was cloned into the pET-29b (Novagen) vector and expressed in E. coli BL21(DE3) cultured in lysogeny medium or minimal medium for 1H/15N-labeled samples.

    SDS Page:

    Article Title: A Drosophila melanogaster model for TMEM43-related arrhythmogenic right ventricular cardiomyopathy type 5.
    Article Snippet: A 402 bp sequence, corresponding to the loop encoding region (aa 34-168), was amplified by PCR and cloned into the pET-29b vector (Novagen, Madison, WI) to generate a His-tag fusion construct.

    Article Title: The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit
    Article Snippet: The PCR products were digested with Nde I and Xho I and inserted into pET-29b vector (Novagen); Escherichia coli BL21(DE3) pLysS cells were transformed with this vector.

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: The tyrosinase-like gene cDNA (Tlg-cDNA) insert was excised from pZL1 (Gibco BRL) with Eco RI and Hind III and recloned into the pET-29b vector (Novagen).

    Article Title: Species Specificity of Vaccinia Virus Complement Control Protein for the Bovine Classical Pathway Is Governed Primarily by Direct Interaction of Its Acidic Residues with Factor I
    Article Snippet: All the mutants were subcloned into the pET-29b vector (Novagen) and transformed into Escherichia coli BL21 cells for expression.

    Article Title: N-Terminal Segment of Tv CyP2 Cyclophilin from Trichomonas vaginalis Is Involved in Self-Association, Membrane Interaction, and Subcellular Localization
    Article Snippet: Tv CyP2 and Tv CyP2-∆N were cloned into the pET-29b vector (Novagen, Darmstadt, Germany) by using NdeI and XhoI restriction sites and were expressed in E. coli strain BL21(DE3) with an additional His-tag at the C-terminus [ ].

    Article Title: Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization
    Article Snippet: Full-length cDNA for NLP, Nph, and TAP/p32 (LD16456-, SD23244-, and LD29590-expressed sequences tags, respectively) were amplified by PCR and cloned in-frame with C-terminal 6-His tag into pET-29b vector (Novagen).

    Article Title: Functional analysis of the GmESR1 gene associated with soybean regeneration
    Article Snippet: The PCR products were digested with Bam HI and Hin dIII and were inserted into the pET-29b vector (EMD Millipore, USA).

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis.
    Article Snippet: The gene encoding Myb135–141 was cloned into the pET-29b (Novagen) vector and expressed in E. coli BL21(DE3) cultured in lysogeny medium or minimal medium for 1H/15N-labeled samples.

    In Vitro:

    Article Title: A Drosophila melanogaster model for TMEM43-related arrhythmogenic right ventricular cardiomyopathy type 5.
    Article Snippet: A 402 bp sequence, corresponding to the loop encoding region (aa 34-168), was amplified by PCR and cloned into the pET-29b vector (Novagen, Madison, WI) to generate a His-tag fusion construct.

    Article Title: The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit
    Article Snippet: The PCR products were digested with Nde I and Xho I and inserted into pET-29b vector (Novagen); Escherichia coli BL21(DE3) pLysS cells were transformed with this vector.

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: The tyrosinase-like gene cDNA (Tlg-cDNA) insert was excised from pZL1 (Gibco BRL) with Eco RI and Hind III and recloned into the pET-29b vector (Novagen).

    Article Title: Species Specificity of Vaccinia Virus Complement Control Protein for the Bovine Classical Pathway Is Governed Primarily by Direct Interaction of Its Acidic Residues with Factor I
    Article Snippet: All the mutants were subcloned into the pET-29b vector (Novagen) and transformed into Escherichia coli BL21 cells for expression.

    Article Title: N-Terminal Segment of Tv CyP2 Cyclophilin from Trichomonas vaginalis Is Involved in Self-Association, Membrane Interaction, and Subcellular Localization
    Article Snippet: Tv CyP2 and Tv CyP2-∆N were cloned into the pET-29b vector (Novagen, Darmstadt, Germany) by using NdeI and XhoI restriction sites and were expressed in E. coli strain BL21(DE3) with an additional His-tag at the C-terminus [ ].

    Article Title: Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization
    Article Snippet: Full-length cDNA for NLP, Nph, and TAP/p32 (LD16456-, SD23244-, and LD29590-expressed sequences tags, respectively) were amplified by PCR and cloned in-frame with C-terminal 6-His tag into pET-29b vector (Novagen).

    Article Title: Functional analysis of the GmESR1 gene associated with soybean regeneration
    Article Snippet: The PCR products were digested with Bam HI and Hin dIII and were inserted into the pET-29b vector (EMD Millipore, USA).

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis.
    Article Snippet: The gene encoding Myb135–141 was cloned into the pET-29b (Novagen) vector and expressed in E. coli BL21(DE3) cultured in lysogeny medium or minimal medium for 1H/15N-labeled samples.

    Sequencing:

    Article Title: A Drosophila melanogaster model for TMEM43-related arrhythmogenic right ventricular cardiomyopathy type 5.
    Article Snippet: A 402 bp sequence, corresponding to the loop encoding region (aa 34-168), was amplified by PCR and cloned into the pET-29b vector (Novagen, Madison, WI) to generate a His-tag fusion construct.

    Article Title: The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit
    Article Snippet: The PCR products were digested with Nde I and Xho I and inserted into pET-29b vector (Novagen); Escherichia coli BL21(DE3) pLysS cells were transformed with this vector.

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: The tyrosinase-like gene cDNA (Tlg-cDNA) insert was excised from pZL1 (Gibco BRL) with Eco RI and Hind III and recloned into the pET-29b vector (Novagen).

    Article Title: Species Specificity of Vaccinia Virus Complement Control Protein for the Bovine Classical Pathway Is Governed Primarily by Direct Interaction of Its Acidic Residues with Factor I
    Article Snippet: All the mutants were subcloned into the pET-29b vector (Novagen) and transformed into Escherichia coli BL21 cells for expression.

    Article Title: N-Terminal Segment of Tv CyP2 Cyclophilin from Trichomonas vaginalis Is Involved in Self-Association, Membrane Interaction, and Subcellular Localization
    Article Snippet: Tv CyP2 and Tv CyP2-∆N were cloned into the pET-29b vector (Novagen, Darmstadt, Germany) by using NdeI and XhoI restriction sites and were expressed in E. coli strain BL21(DE3) with an additional His-tag at the C-terminus [ ].

    Article Title: Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization
    Article Snippet: Full-length cDNA for NLP, Nph, and TAP/p32 (LD16456-, SD23244-, and LD29590-expressed sequences tags, respectively) were amplified by PCR and cloned in-frame with C-terminal 6-His tag into pET-29b vector (Novagen).

    Article Title: Functional analysis of the GmESR1 gene associated with soybean regeneration
    Article Snippet: The PCR products were digested with Bam HI and Hin dIII and were inserted into the pET-29b vector (EMD Millipore, USA).

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis.
    Article Snippet: The gene encoding Myb135–141 was cloned into the pET-29b (Novagen) vector and expressed in E. coli BL21(DE3) cultured in lysogeny medium or minimal medium for 1H/15N-labeled samples.

    Amplification:

    Article Title: A Drosophila melanogaster model for TMEM43-related arrhythmogenic right ventricular cardiomyopathy type 5.
    Article Snippet: A 402 bp sequence, corresponding to the loop encoding region (aa 34-168), was amplified by PCR and cloned into the pET-29b vector (Novagen, Madison, WI) to generate a His-tag fusion construct.

    Article Title: The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit
    Article Snippet: The PCR products were digested with Nde I and Xho I and inserted into pET-29b vector (Novagen); Escherichia coli BL21(DE3) pLysS cells were transformed with this vector.

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: The tyrosinase-like gene cDNA (Tlg-cDNA) insert was excised from pZL1 (Gibco BRL) with Eco RI and Hind III and recloned into the pET-29b vector (Novagen).

    Article Title: Species Specificity of Vaccinia Virus Complement Control Protein for the Bovine Classical Pathway Is Governed Primarily by Direct Interaction of Its Acidic Residues with Factor I
    Article Snippet: All the mutants were subcloned into the pET-29b vector (Novagen) and transformed into Escherichia coli BL21 cells for expression.

    Article Title: N-Terminal Segment of Tv CyP2 Cyclophilin from Trichomonas vaginalis Is Involved in Self-Association, Membrane Interaction, and Subcellular Localization
    Article Snippet: Tv CyP2 and Tv CyP2-∆N were cloned into the pET-29b vector (Novagen, Darmstadt, Germany) by using NdeI and XhoI restriction sites and were expressed in E. coli strain BL21(DE3) with an additional His-tag at the C-terminus [ ].

    Article Title: Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization
    Article Snippet: Full-length cDNA for NLP, Nph, and TAP/p32 (LD16456-, SD23244-, and LD29590-expressed sequences tags, respectively) were amplified by PCR and cloned in-frame with C-terminal 6-His tag into pET-29b vector (Novagen).

    Article Title: Functional analysis of the GmESR1 gene associated with soybean regeneration
    Article Snippet: The PCR products were digested with Bam HI and Hin dIII and were inserted into the pET-29b vector (EMD Millipore, USA).

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis.
    Article Snippet: The gene encoding Myb135–141 was cloned into the pET-29b (Novagen) vector and expressed in E. coli BL21(DE3) cultured in lysogeny medium or minimal medium for 1H/15N-labeled samples.

    Polymerase Chain Reaction:

    Article Title: A Drosophila melanogaster model for TMEM43-related arrhythmogenic right ventricular cardiomyopathy type 5.
    Article Snippet: A 402 bp sequence, corresponding to the loop encoding region (aa 34-168), was amplified by PCR and cloned into the pET-29b vector (Novagen, Madison, WI) to generate a His-tag fusion construct.

    Article Title: The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit
    Article Snippet: The PCR products were digested with Nde I and Xho I and inserted into pET-29b vector (Novagen); Escherichia coli BL21(DE3) pLysS cells were transformed with this vector.

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: The tyrosinase-like gene cDNA (Tlg-cDNA) insert was excised from pZL1 (Gibco BRL) with Eco RI and Hind III and recloned into the pET-29b vector (Novagen).

    Article Title: Species Specificity of Vaccinia Virus Complement Control Protein for the Bovine Classical Pathway Is Governed Primarily by Direct Interaction of Its Acidic Residues with Factor I
    Article Snippet: All the mutants were subcloned into the pET-29b vector (Novagen) and transformed into Escherichia coli BL21 cells for expression.

    Article Title: N-Terminal Segment of Tv CyP2 Cyclophilin from Trichomonas vaginalis Is Involved in Self-Association, Membrane Interaction, and Subcellular Localization
    Article Snippet: Tv CyP2 and Tv CyP2-∆N were cloned into the pET-29b vector (Novagen, Darmstadt, Germany) by using NdeI and XhoI restriction sites and were expressed in E. coli strain BL21(DE3) with an additional His-tag at the C-terminus [ ].

    Article Title: Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization
    Article Snippet: Full-length cDNA for NLP, Nph, and TAP/p32 (LD16456-, SD23244-, and LD29590-expressed sequences tags, respectively) were amplified by PCR and cloned in-frame with C-terminal 6-His tag into pET-29b vector (Novagen).

    Article Title: Functional analysis of the GmESR1 gene associated with soybean regeneration
    Article Snippet: The PCR products were digested with Bam HI and Hin dIII and were inserted into the pET-29b vector (EMD Millipore, USA).

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis.
    Article Snippet: The gene encoding Myb135–141 was cloned into the pET-29b (Novagen) vector and expressed in E. coli BL21(DE3) cultured in lysogeny medium or minimal medium for 1H/15N-labeled samples.

    Clone Assay:

    Article Title: A Drosophila melanogaster model for TMEM43-related arrhythmogenic right ventricular cardiomyopathy type 5.
    Article Snippet: A 402 bp sequence, corresponding to the loop encoding region (aa 34-168), was amplified by PCR and cloned into the pET-29b vector (Novagen, Madison, WI) to generate a His-tag fusion construct.

    Article Title: The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit
    Article Snippet: The PCR products were digested with Nde I and Xho I and inserted into pET-29b vector (Novagen); Escherichia coli BL21(DE3) pLysS cells were transformed with this vector.

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: The tyrosinase-like gene cDNA (Tlg-cDNA) insert was excised from pZL1 (Gibco BRL) with Eco RI and Hind III and recloned into the pET-29b vector (Novagen).

    Article Title: Species Specificity of Vaccinia Virus Complement Control Protein for the Bovine Classical Pathway Is Governed Primarily by Direct Interaction of Its Acidic Residues with Factor I
    Article Snippet: All the mutants were subcloned into the pET-29b vector (Novagen) and transformed into Escherichia coli BL21 cells for expression.

    Article Title: N-Terminal Segment of Tv CyP2 Cyclophilin from Trichomonas vaginalis Is Involved in Self-Association, Membrane Interaction, and Subcellular Localization
    Article Snippet: Tv CyP2 and Tv CyP2-∆N were cloned into the pET-29b vector (Novagen, Darmstadt, Germany) by using NdeI and XhoI restriction sites and were expressed in E. coli strain BL21(DE3) with an additional His-tag at the C-terminus [ ].

    Article Title: Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization
    Article Snippet: Full-length cDNA for NLP, Nph, and TAP/p32 (LD16456-, SD23244-, and LD29590-expressed sequences tags, respectively) were amplified by PCR and cloned in-frame with C-terminal 6-His tag into pET-29b vector (Novagen).

    Article Title: Functional analysis of the GmESR1 gene associated with soybean regeneration
    Article Snippet: The PCR products were digested with Bam HI and Hin dIII and were inserted into the pET-29b vector (EMD Millipore, USA).

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis.
    Article Snippet: The gene encoding Myb135–141 was cloned into the pET-29b (Novagen) vector and expressed in E. coli BL21(DE3) cultured in lysogeny medium or minimal medium for 1H/15N-labeled samples.

    Construct:

    Article Title: A Drosophila melanogaster model for TMEM43-related arrhythmogenic right ventricular cardiomyopathy type 5.
    Article Snippet: A 402 bp sequence, corresponding to the loop encoding region (aa 34-168), was amplified by PCR and cloned into the pET-29b vector (Novagen, Madison, WI) to generate a His-tag fusion construct.

    Article Title: The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit
    Article Snippet: The PCR products were digested with Nde I and Xho I and inserted into pET-29b vector (Novagen); Escherichia coli BL21(DE3) pLysS cells were transformed with this vector.

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: The tyrosinase-like gene cDNA (Tlg-cDNA) insert was excised from pZL1 (Gibco BRL) with Eco RI and Hind III and recloned into the pET-29b vector (Novagen).

    Article Title: Species Specificity of Vaccinia Virus Complement Control Protein for the Bovine Classical Pathway Is Governed Primarily by Direct Interaction of Its Acidic Residues with Factor I
    Article Snippet: All the mutants were subcloned into the pET-29b vector (Novagen) and transformed into Escherichia coli BL21 cells for expression.

    Article Title: N-Terminal Segment of Tv CyP2 Cyclophilin from Trichomonas vaginalis Is Involved in Self-Association, Membrane Interaction, and Subcellular Localization
    Article Snippet: Tv CyP2 and Tv CyP2-∆N were cloned into the pET-29b vector (Novagen, Darmstadt, Germany) by using NdeI and XhoI restriction sites and were expressed in E. coli strain BL21(DE3) with an additional His-tag at the C-terminus [ ].

    Article Title: Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization
    Article Snippet: Full-length cDNA for NLP, Nph, and TAP/p32 (LD16456-, SD23244-, and LD29590-expressed sequences tags, respectively) were amplified by PCR and cloned in-frame with C-terminal 6-His tag into pET-29b vector (Novagen).

    Article Title: Functional analysis of the GmESR1 gene associated with soybean regeneration
    Article Snippet: The PCR products were digested with Bam HI and Hin dIII and were inserted into the pET-29b vector (EMD Millipore, USA).

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis.
    Article Snippet: The gene encoding Myb135–141 was cloned into the pET-29b (Novagen) vector and expressed in E. coli BL21(DE3) cultured in lysogeny medium or minimal medium for 1H/15N-labeled samples.

    Cell Culture:

    Article Title: A Drosophila melanogaster model for TMEM43-related arrhythmogenic right ventricular cardiomyopathy type 5.
    Article Snippet: A 402 bp sequence, corresponding to the loop encoding region (aa 34-168), was amplified by PCR and cloned into the pET-29b vector (Novagen, Madison, WI) to generate a His-tag fusion construct.

    Article Title: The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit
    Article Snippet: The PCR products were digested with Nde I and Xho I and inserted into pET-29b vector (Novagen); Escherichia coli BL21(DE3) pLysS cells were transformed with this vector.

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: The tyrosinase-like gene cDNA (Tlg-cDNA) insert was excised from pZL1 (Gibco BRL) with Eco RI and Hind III and recloned into the pET-29b vector (Novagen).

    Article Title: Species Specificity of Vaccinia Virus Complement Control Protein for the Bovine Classical Pathway Is Governed Primarily by Direct Interaction of Its Acidic Residues with Factor I
    Article Snippet: All the mutants were subcloned into the pET-29b vector (Novagen) and transformed into Escherichia coli BL21 cells for expression.

    Article Title: N-Terminal Segment of Tv CyP2 Cyclophilin from Trichomonas vaginalis Is Involved in Self-Association, Membrane Interaction, and Subcellular Localization
    Article Snippet: Tv CyP2 and Tv CyP2-∆N were cloned into the pET-29b vector (Novagen, Darmstadt, Germany) by using NdeI and XhoI restriction sites and were expressed in E. coli strain BL21(DE3) with an additional His-tag at the C-terminus [ ].

    Article Title: Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization
    Article Snippet: Full-length cDNA for NLP, Nph, and TAP/p32 (LD16456-, SD23244-, and LD29590-expressed sequences tags, respectively) were amplified by PCR and cloned in-frame with C-terminal 6-His tag into pET-29b vector (Novagen).

    Article Title: Functional analysis of the GmESR1 gene associated with soybean regeneration
    Article Snippet: The PCR products were digested with Bam HI and Hin dIII and were inserted into the pET-29b vector (EMD Millipore, USA).

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis.
    Article Snippet: The gene encoding Myb135–141 was cloned into the pET-29b (Novagen) vector and expressed in E. coli BL21(DE3) cultured in lysogeny medium or minimal medium for 1H/15N-labeled samples.

    Transformation Assay:

    Article Title: A Drosophila melanogaster model for TMEM43-related arrhythmogenic right ventricular cardiomyopathy type 5.
    Article Snippet: A 402 bp sequence, corresponding to the loop encoding region (aa 34-168), was amplified by PCR and cloned into the pET-29b vector (Novagen, Madison, WI) to generate a His-tag fusion construct.

    Article Title: The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit
    Article Snippet: The PCR products were digested with Nde I and Xho I and inserted into pET-29b vector (Novagen); Escherichia coli BL21(DE3) pLysS cells were transformed with this vector.

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: The tyrosinase-like gene cDNA (Tlg-cDNA) insert was excised from pZL1 (Gibco BRL) with Eco RI and Hind III and recloned into the pET-29b vector (Novagen).

    Article Title: Species Specificity of Vaccinia Virus Complement Control Protein for the Bovine Classical Pathway Is Governed Primarily by Direct Interaction of Its Acidic Residues with Factor I
    Article Snippet: All the mutants were subcloned into the pET-29b vector (Novagen) and transformed into Escherichia coli BL21 cells for expression.

    Article Title: N-Terminal Segment of Tv CyP2 Cyclophilin from Trichomonas vaginalis Is Involved in Self-Association, Membrane Interaction, and Subcellular Localization
    Article Snippet: Tv CyP2 and Tv CyP2-∆N were cloned into the pET-29b vector (Novagen, Darmstadt, Germany) by using NdeI and XhoI restriction sites and were expressed in E. coli strain BL21(DE3) with an additional His-tag at the C-terminus [ ].

    Article Title: Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization
    Article Snippet: Full-length cDNA for NLP, Nph, and TAP/p32 (LD16456-, SD23244-, and LD29590-expressed sequences tags, respectively) were amplified by PCR and cloned in-frame with C-terminal 6-His tag into pET-29b vector (Novagen).

    Article Title: Functional analysis of the GmESR1 gene associated with soybean regeneration
    Article Snippet: The PCR products were digested with Bam HI and Hin dIII and were inserted into the pET-29b vector (EMD Millipore, USA).

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis.
    Article Snippet: The gene encoding Myb135–141 was cloned into the pET-29b (Novagen) vector and expressed in E. coli BL21(DE3) cultured in lysogeny medium or minimal medium for 1H/15N-labeled samples.

    Expressing:

    Article Title: A Drosophila melanogaster model for TMEM43-related arrhythmogenic right ventricular cardiomyopathy type 5.
    Article Snippet: A 402 bp sequence, corresponding to the loop encoding region (aa 34-168), was amplified by PCR and cloned into the pET-29b vector (Novagen, Madison, WI) to generate a His-tag fusion construct.

    Article Title: The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit
    Article Snippet: The PCR products were digested with Nde I and Xho I and inserted into pET-29b vector (Novagen); Escherichia coli BL21(DE3) pLysS cells were transformed with this vector.

    Article Title: A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein
    Article Snippet: The tyrosinase-like gene cDNA (Tlg-cDNA) insert was excised from pZL1 (Gibco BRL) with Eco RI and Hind III and recloned into the pET-29b vector (Novagen).

    Article Title: Species Specificity of Vaccinia Virus Complement Control Protein for the Bovine Classical Pathway Is Governed Primarily by Direct Interaction of Its Acidic Residues with Factor I
    Article Snippet: All the mutants were subcloned into the pET-29b vector (Novagen) and transformed into Escherichia coli BL21 cells for expression.

    Article Title: N-Terminal Segment of Tv CyP2 Cyclophilin from Trichomonas vaginalis Is Involved in Self-Association, Membrane Interaction, and Subcellular Localization
    Article Snippet: Tv CyP2 and Tv CyP2-∆N were cloned into the pET-29b vector (Novagen, Darmstadt, Germany) by using NdeI and XhoI restriction sites and were expressed in E. coli strain BL21(DE3) with an additional His-tag at the C-terminus [ ].

    Article Title: Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization
    Article Snippet: Full-length cDNA for NLP, Nph, and TAP/p32 (LD16456-, SD23244-, and LD29590-expressed sequences tags, respectively) were amplified by PCR and cloned in-frame with C-terminal 6-His tag into pET-29b vector (Novagen).

    Article Title: Functional analysis of the GmESR1 gene associated with soybean regeneration
    Article Snippet: The PCR products were digested with Bam HI and Hin dIII and were inserted into the pET-29b vector (EMD Millipore, USA).

    Article Title: Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis.
    Article Snippet: The gene encoding Myb135–141 was cloned into the pET-29b (Novagen) vector and expressed in E. coli BL21(DE3) cultured in lysogeny medium or minimal medium for 1H/15N-labeled samples.



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